RPMI 8226/GFP-Firefly Luciferase Cell Line
Description: RPMI 8226 is a human multiple myeloma cell line originally derived from a patient with plasmacytoma. These cells constitutively express BCMA and CD38, making them a physiologically relevant model for evaluating cancer immunotherapies, including Chimeric Antigen Receptor (CAR) T cells. The RPMI 8226/GFP-firefly luciferase variant stably expresses both GFP and firefly luciferase, enabling use in in vitro cytotoxicity assays and in vivo studies.
Quantity: One vial of frozen cells (2×106 per vial)
Stability: Stable in culture over a minimum of 10 passages
Application: Drug screening, biological assays and in vivo study
Freezing Medium: 7% RPMI 1640 +20% FBS+10% DMSO
Propagation Medium: RPMI1640+10%FBS
Morphology: Lymphoblast
Subculture: Start cultures 5.0 X 105 cells/mL maintain between 1.0 X 105 and 2.0 X 106 cells/mL.
Incubation: 37 °C with 5% CO2
Storage: Liquid nitrogen immediately upon receiving
Doubling Time: Approximately 26 hours
Mycoplasma Status: Negative
Caution: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Description: RPMI 8226 is a human multiple myeloma cell line originally derived from a patient with plasmacytoma. These cells constitutively express BCMA and CD38, making them a physiologically relevant model for evaluating cancer immunotherapies, including Chimeric Antigen Receptor (CAR) T cells. The RPMI 8226/GFP-firefly luciferase variant stably expresses both GFP and firefly luciferase, enabling use in in vitro cytotoxicity assays and in vivo studies.
Quantity: One vial of frozen cells (2×106 per vial)
Stability: Stable in culture over a minimum of 10 passages
Application: Drug screening, biological assays and in vivo study
Freezing Medium: 7% RPMI 1640 +20% FBS+10% DMSO
Propagation Medium: RPMI1640+10%FBS
Morphology: Lymphoblast
Subculture: Start cultures 5.0 X 105 cells/mL maintain between 1.0 X 105 and 2.0 X 106 cells/mL.
Incubation: 37 °C with 5% CO2
Storage: Liquid nitrogen immediately upon receiving
Doubling Time: Approximately 26 hours
Mycoplasma Status: Negative
Caution: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.