Immunotherapy is transforming cancer treatment, with CAR-T cell therapy emerging as a groundbreaking approach for hematologic malignancies such as leukemia and lymphoma. To expand its therapeutic reach, researchers are developing allogeneic (off-the-shelf) CAR-T cells by disrupting genes like TCR to prevent graft-versus-host disease (GvHD).

In parallel, CAR-engineered natural killer (CAR-NK) cells are gaining momentum as an alternative platform. Unlike T cells, NK cells do not require prior activation and can recognize tumor cells independent of MHC expression—making them ideal for universal, allogeneic applications. CAR-NK therapies also tend to have a more favorable safety profile, with reduced risk of cytokine release syndrome (CRS) and neurotoxicity.

With deep expertise in tumor immunology, RedyBio provides comprehensive CRO services to accelerate your CAR-T, CAR-NK, and other immune cell therapy programs. Our services include:

• Monoclonal antibody development

• CAR construct design and vector production

• CAR lentivirus/Retrovirus manufacturing

• Immune cell isolation, activation, and transduction

• Phenotypic and functional analysis

• In vitro cytotoxicity assays

• In vivo efficacy models

RedyBio Custom Immune Cell Therapy Research Services

Accelerate your immunotherapy development with RedyBio’s end-to-end support for CAR-T, CAR-NK, and other immune cell-based research. Our comprehensive services span antibody discovery to in vivo efficacy evaluation.

Antibody Development

• Animal immunization

• Hybridoma generation and screening

• Subcloning and large-scale amplification

• Hybridoma sequencing

• Functional assay validation

CAR Design & Lentivirus Production

• Custom CAR molecule design and vector construction

• High-titer CAR lentiviral/retroviral vector production

• Stable cell line generation for target antigen expression

Immune Cell Preparation

• Human T and CAR-T cell preparation

• NK and CAR-NK cell generation

• Allogeneic (“universal”) CAR-T cell production

• Murine CAR-T cell services

Phenotypic Characterization

• T cell analysis: activation, exhaustion, memory subsets

• NK cell surface marker profiling

• Myeloid and other immune cell phenotyping

In Vitro Efficacy Assays

• CAR-T/NK cell proliferation assays

• Target cell cytotoxicity analysis

• Chronic antigen stimulation assay

• ADCC (antibody-dependent cellular cytotoxicity) assays

• ADCP (antibody-dependent cellular phagocytosis) assays

• Multiplex cytokine detection

In Vivo Efficacy Studies

• CDX (cell line-derived xenograft) tumor models using immunodeficient mice

• Syngeneic mouse tumor models

• Humanized immune system models (PBMC and HSC-based)

• Dosing studies and tumor growth monitoring

• Biochemical and histological analysis of tissues and samples